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tubulin polymerization kit  (Cytoskeleton Inc)
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Cytoskeleton Inc tubulin polymerization kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
Tubulin Polymerization Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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progesterone accubind elisa test system kit  (Monobind)
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Monobind progesterone accubind elisa test system kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
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io test beta mark tcr beta repertoire kit  (Danaher Inc)
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Danaher Inc io test beta mark tcr beta repertoire kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
Io Test Beta Mark Tcr Beta Repertoire Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucose c-ii test kit  (FUJIFILM)
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FUJIFILM glucose c-ii test kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
Glucose C Ii Test Kit, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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snap β-lactam test kit  (IDEXX)
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IDEXX snap β-lactam test kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
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glucose cii-test  (FUJIFILM)
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Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
Glucose Cii Test, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemiluminescence immunoassay test kit advia centaur progesterone assay  (Siemens AG)
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Siemens AG chemiluminescence immunoassay test kit advia centaur progesterone assay
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
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quantikine human tgf β1 kit  (R&D Systems)
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R&D Systems quantikine human tgf β1 kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
Quantikine Human Tgf β1 Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β gal galactosidase detection kit  (Danaher Inc)
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Danaher Inc β gal galactosidase detection kit
Parbendazole inhibits <t>tubulin</t> <t>polymerization.</t> (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).
β Gal Galactosidase Detection Kit, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il 6  (Danaher Inc)
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Danaher Inc il 6
Figure 5. Effects of tanshinone IIA-NP on inflammatory factors after MI. (A) The mRNA level of inflammatory factors were analyzed by Real-time PCR in LV ischemic border zone 4 weeks after ligation. Data are shown as the ratio of mRNA level of each factors relative to 18S mRNA and expressed as the fold change relative to the mean value of sham-operated mice (n ¼ 6). (B) <t>ELISA</t> assay to quantify the production of the inflammatory factors by the hearts after LAD ligation.(C) Representative immunohistochemical photographs and quantification assay of the ischemic border zone 4 weeks after LAD ligation. #p < .05 versus Sham; p < .05 versus MI.
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advia centaur testosterone test kit  (Siemens AG)
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Figure 5. Effects of tanshinone IIA-NP on inflammatory factors after MI. (A) The mRNA level of inflammatory factors were analyzed by Real-time PCR in LV ischemic border zone 4 weeks after ligation. Data are shown as the ratio of mRNA level of each factors relative to 18S mRNA and expressed as the fold change relative to the mean value of sham-operated mice (n ¼ 6). (B) <t>ELISA</t> assay to quantify the production of the inflammatory factors by the hearts after LAD ligation.(C) Representative immunohistochemical photographs and quantification assay of the ischemic border zone 4 weeks after LAD ligation. #p < .05 versus Sham; p < .05 versus MI.
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urinary free cortisol test-kit dko018  (Diametra SRL)
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Figure 5. Effects of tanshinone IIA-NP on inflammatory factors after MI. (A) The mRNA level of inflammatory factors were analyzed by Real-time PCR in LV ischemic border zone 4 weeks after ligation. Data are shown as the ratio of mRNA level of each factors relative to 18S mRNA and expressed as the fold change relative to the mean value of sham-operated mice (n ¼ 6). (B) <t>ELISA</t> assay to quantify the production of the inflammatory factors by the hearts after LAD ligation.(C) Representative immunohistochemical photographs and quantification assay of the ischemic border zone 4 weeks after LAD ligation. #p < .05 versus Sham; p < .05 versus MI.
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Image Search Results


Parbendazole inhibits tubulin polymerization. (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).

Journal: Acta Pharmaceutica Sinica. B

Article Title: Identification of anthelmintic parbendazole as a therapeutic molecule for HNSCC through connectivity map-based drug repositioning

doi: 10.1016/j.apsb.2021.12.005

Figure Lengend Snippet: Parbendazole inhibits tubulin polymerization. (A) is the result of immunofluorescence assay which displays the microtubule structure of cells. Red (Alexa Fluor® 555) was used to mark tubulin, and blue (DAPI) was used to mark cell nuclei. Images were obtained by Laser Confocal Microscope (LSM780). The scale bar was 20 μm; (B) shows compounds inhibited tubulin polymerization in vitro . The spontaneous polymerization of tubulin in the absence of the compound was used as a negative control; (C) is the result of Western blotting of the amount of unpolymerizated and polymerizated tubulin; (D) shows unpolymerizated/polymerizated tubulin ratio. Data is represented as mean ± SEM ( n = 3, ∗ P < 0.05).

Article Snippet: This test was performed using the tubulin polymerization kit in accordance with the manufacturer's protocol (BK011P, Cytoskeleton, Inc.).

Techniques: Immunofluorescence, Microscopy, In Vitro, Negative Control, Western Blot

Figure 5. Effects of tanshinone IIA-NP on inflammatory factors after MI. (A) The mRNA level of inflammatory factors were analyzed by Real-time PCR in LV ischemic border zone 4 weeks after ligation. Data are shown as the ratio of mRNA level of each factors relative to 18S mRNA and expressed as the fold change relative to the mean value of sham-operated mice (n ¼ 6). (B) ELISA assay to quantify the production of the inflammatory factors by the hearts after LAD ligation.(C) Representative immunohistochemical photographs and quantification assay of the ischemic border zone 4 weeks after LAD ligation. #p < .05 versus Sham; p < .05 versus MI.

Journal: Artificial cells, nanomedicine, and biotechnology

Article Title: Nanoparticle-mediated delivery of Tanshinone IIA reduces adverse cardiac remodeling following myocardial infarctions in a mice model: role of NF-κB pathway.

doi: 10.1080/21691401.2018.1508028

Figure Lengend Snippet: Figure 5. Effects of tanshinone IIA-NP on inflammatory factors after MI. (A) The mRNA level of inflammatory factors were analyzed by Real-time PCR in LV ischemic border zone 4 weeks after ligation. Data are shown as the ratio of mRNA level of each factors relative to 18S mRNA and expressed as the fold change relative to the mean value of sham-operated mice (n ¼ 6). (B) ELISA assay to quantify the production of the inflammatory factors by the hearts after LAD ligation.(C) Representative immunohistochemical photographs and quantification assay of the ischemic border zone 4 weeks after LAD ligation. #p < .05 versus Sham; p < .05 versus MI.

Article Snippet: Mouse MCP1 (ab208979), TNFa (ab208348), IL-1b (ab100704), IL-6 (ab100712), IL-18 (ab216165) ELISA Kit were purchased from Abcam (Cambridge, MA, USA).

Techniques: Real-time Polymerase Chain Reaction, Ligation, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining